Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri
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چکیده
منابع مشابه
Generation of Doubled Haploid Transgenic Wheat Lines by Microspore Transformation
Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Di...
متن کاملبررسی اثر ژنوتیپ، پیش‌تیمارتنشی و محیط‌کشت در باززایی میکروسپورهای جو از طریق کشت بساک
Doubled haploid production is one of the best breeding methods to produce homozygous (pure) lines. Microspore culture technique is the most efficient method for production of doubled haploid plants. This research was carried out to study the effect of genotypes, stress pre-treatments and culture media on the callus induction and plant regeneration from microspores of anthers in barley (Hordeum ...
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Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...
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haploid plant per floret (Devaux, 1988). Androgenesis induction in microspores may be affected by various An efficient doubled-haploid production technology that induces factors which cause low induction efficiency and by gehomozygosity can greatly reduce the time and cost of cultivar developnotype dependence (Dunwell, 1985). Most advances toment. Low efficiency of doubled-haploid production pr...
متن کاملHaploid mouse embryos obtained by microsurgical removal of one pronucleus.
A pronucleus can be microsurgically removed from the fertilized mouse egg. Out of 145 haploid eggs obtained by this method and transplanted into the oviduct of pseudopregnant recipients, 36 multicellular embryos were recovered on the 4th or 5th day. On the 4th day all embryos were morulae composed of 8-50 cells, with the majority containing 8-16 cells. After an additional 24 h in vivo or in vit...
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ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 1999
ISSN: 0721-7714,1432-203X
DOI: 10.1007/s002990050685